Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
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چکیده
منابع مشابه
Preparative SDS-PAGE Electroelution for Rapid Purification of Alkyl Hydroperoxide Reductase from Helicobacter pylori
BACKGROUND Alkyl hydroperoxide reductase (AhpC) of Helicobacter pylori is considered as a diagnostic antigen. Therefore, this antigen can be used to detect H. pylori infection by stool immunoassays such as ELISA. The aim of this study was to simplify the AhpC protein purification procedures. METHODS For whole cell protein extraction, the bacterial cells were ruptured by octly-β-D glucopyranos...
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Recombinant protein technology provides an invaluable source of proteins for use in structure-function studies, as immunogens, and in the development of therapeutics. Recombinant proteins are typically engineered with "tags" that allow the protein to be purified from crude host cell extracts using affinity based chromatography techniques. Amelogenin is the principal component of the developing ...
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INTRODUCTION MATERIALS AND METHODS RESULTS AND DISCUSSION CONCLUSIONS REFERENCES
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Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...
متن کاملpreparative sds-page electroelution for rapid purification of alkyl hydroperoxide reductase from helicobacter pylori
background: alkyl hydroperoxide reductase (ahpc) of helicobacter pylori is considered as a diagnostic antigen. therefore, this antigen can be used to detect h. pylori infection by stool immunoassays such as elisa. the aim of this study was to simplify the ahpc protein purification procedures. methods: for whole cell protein extraction, the bacterial cells were ruptured by octly-β-d glucopyrano...
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ژورنال
عنوان ژورنال: The Scientific World JOURNAL
سال: 2003
ISSN: 1537-744X
DOI: 10.1100/tsw.2003.30